Sains Malaysiana 53(10)(2024): 3263-3277

http://doi.org/10.17576/jsm-2024-5310-03

Sistematik Famili Athyriaceae di Semenanjung Malaysia Berdasarkan Gabungan Data Anatomi dan Molekul

(Systematics of Athyriaceae Family in Peninsular Malaysia Based on Combination Anatomical and Molecular Data)

NUR ALIAH*, HAJA MAIDEEN, NURUL NADHIRAH & NIK NORHAZRINA

Jabatan Sains Biologi dan Bioteknologi, Fakulti Sains dan Teknologi, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor, Malaysia

Diserahkan: 5 Mei 2024/Diterima: 10 Julai 2024

Abstrak

Famili paku pakis Athyriaceae mempunyai taburan kosmopolitan dengan anggaran 650 takson. Pengelasan terkini berdasarkan data daripada jujukan molekul telah mengelaskan spesies Athyriaceae kepada tiga genus utama iaitu Athyrium, Deparia serta Diplazium. Walau bagaimanapun, pengelasan ini masih tidak stabil dan sering berubah kerana belum terdapat data jujukan molekul yang lengkap serta menyeluruh bagi keseluruhan spesies dalam famili ini. Penyelidikan ini telah dijalankan untuk mengkaji hubungan filogeni antara spesies dalam famili Athyriaceae di Semenanjung Malaysia berdasarkan gabungan data ciri anatomi dan molekul. Bagi pendekatan anatomi, ciri bentuk stil dan jenis peruratan telah dicerap manakala bagi pendekatan molekul pula menggunakan data jujukan dari kawasan genom kloroplas (rbcL, atpB, atpA dan trnL-F) dan nukleus ITS (nITS). Keseluruhannya, topologi yang terhasil membuktikan bahawa famili Athyriaceae ialah monofiletik dengan sokongannilai bustrap yang tinggi iaitu 100% serta membentuk dua klad utamaiaitu klad I yang terdiri daripada kesemua spesies Diplazium manakala klad II terdiri daripada kumpulan Athyrium-Cornopteris yang membentuk kumpulan bersama dengan Deparia.

Kata kunci: Anatomi; Athyriaceae; Diplazium; paku pakis; sistematik

Abstract

The ferns family Athyriaceae has a cosmopolitan distribution with ca. 650 taxa. Current classification based on molecular data characterized members of Athyriaceae into three main genera which are Athyrium, Deparia, and Diplazium. However, the classification of this family is still unstable due to incomplete molecular data of the species in the family. This study was conducted to study the phylogeny relationships of Athyriaceae species in Peninsular Malaysia based on combination of anatomical and molecular data. In anatomical approach, characters such as stele shape and type of venations have been observed meanwhile for molecular study, sequences data were based on chloroplast regions (rbcL, atpB, atpA, trnL-F) and nuclear ITS (nITS). Overall, the topology produced based on DNA sequences proved Athyriaceae is a monophyletic family and supported well with high bootstrap value 100%. In general, the trees produced formed two main clades in which clade I: consists of all Diplazium species meanwhile clade II comprises of Athyrium-Cornopteris and formed sister group with Deparia.

Keywords: Anatomy; Athyriaceae; Diplazium; ferns; systematics

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*Pengarang untuk surat-menyurat; email: aliahkhaduwi@ukm.edu.my